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Floxing by Electroporating Single-Cell Embryos with Two CRISPR RNPs and Two ssODNs.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2023

Wallace Mia, White J Michael, Kouranova Evgenea, Wang Zi Teng, Cui Xiaoxia

Abstract excerpt

Floxed alleles and Cre drivers are two components of most conditional knockout mouse models, which are not only important for studying a given gene in a tissue-specific manner, but also useful for functional analysis of various sized genomic regions. With the increased demand for floxed mouse models in biomedical research, reliable and economical creation of floxed alleles is clearly highly valuable yet remains...

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PMID: 36995670
DOI: 10.1007/978-1-0716-2990-1_9

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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