Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Genotyping Genome-Edited Founders and Subsequent Generation.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2023

Mackenzie Matthew, Fower Alex, Allan Alasdair J, Codner Gemma F, Bunton-Stasyshyn Rosie K, Teboul Lydia

Abstract excerpt

Targeted nucleases allow the production of many types of genetic mutations directly in the early embryo. However, the outcome of their activity is a repair event of unpredictable nature, and the founder animals that are produced are generally of a mosaic nature. Here, we present the molecular assays and genotyping strategies that will support the screening of the first generation for potential founders and the...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to CRISPR-Cas Systems
Share to Gene Editing
Share to Genome

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Genotype

Share to Mutation

Source

Identifiers

PMID: 36995665
DOI: 10.1007/978-1-0716-2990-1_4

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Genotyping Genome-Edited Founders and Subsequent Generation.PMID 36995665Read ↗

Screening and validation of genome-edited animals.Semantic proximity Read ↗

Analysing the outcome of CRISPR-aided genome editing in embryos: Screening, genotyping and quality control.Semantic proximity Read ↗

Detailed phenotypic and molecular analyses of genetically modified mice generated by CRISPR-Cas9-mediated editing.Semantic proximity Read ↗

Genotyping Protocols for Genetically Engineered Mice.Semantic proximity Read ↗

Long-read sequencing for fast and robust identification of correct genome-edited alleles: PCR-based and Cas9 capture methods.Semantic proximity Read ↗

Recovering a Targeted Mutation in Mice from Embryonic Stem Cell Chimeras or CRISPR-Cas Founders.Semantic proximity Read ↗

Floxing by Electroporating Single-Cell Embryos with Two CRISPR RNPs and Two ssODNs.Semantic proximity Read ↗

Generation of Genetically Modified Mice through the Microinjection of Oocytes.Semantic proximity Read ↗

Genetic engineering as a tool for the generation of mouse models to understand disease phenotypes and gene function.Semantic proximity Read ↗

Super-Mendelian inheritance mediated by CRISPR/Cas9 in the female mouse germlineSemantic proximity Read ↗

Calls grow to tap the gold mine of human genetic knockoutsSemantic proximity Read ↗

Early zygotic engineering promotes targeted large transgene integration and direct production of fully transgenic animalsSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.