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Article

A simple and practical workflow for genotyping of CRISPR-Cas9-based knockout phenotypes using multiplexed amplicon sequencing.

Genes to cells : devoted to molecular & cellular mechanisms - 1 Jul 2020

Iida Midori, Suzuki Miyuki, Sakane Yuto, Nishide Hiroyo, Uchiyama Ikuo, Yamamoto Takashi, Suzuki Ken-Ichi T, Fujii Satoshi

Abstract excerpt

Founder animals carrying high proportions of somatic mutation induced by CRISPR-Cas9 enable a rapid and scalable strategy for the functional screening of numerous target genes in vivo. In this functional screening, genotyping using pooled amplicons with next-generation sequencing is the most suitable approach for large-scale management of multiple samples and accurate evaluation of the efficiency of Cas9-induced...

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PMID: 32323394
DOI: 10.1111/gtc.12775

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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