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Experimental Validation of RNA-RNA Interactions by Electrophoretic Mobility Shift Assay.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2024

Lillebæk Eva Maria Sternkopf, Kallipolitis Birgitte Haahr

Abstract excerpt

Regulatory RNAs in bacteria are known to act by base pairing with other RNAs. Interactions between two partner RNAs can be investigated by electrophoretic mobility shift assays. The regions predicted to be engaged in base pairing are analyzed by introducing mutations in one RNA that prevent RNA-RNA complex formation. Next, base pairing is restored by introducing complementary mutations in its partner RNA. Here,...

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PMID: 38217655
DOI: 10.1007/978-1-0716-3565-0_11

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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