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Article

Use of electrophoretic mobility to determine the secondary structure of a small antisense RNA.

Nucleic acids research - 11 Jun 1991

Jacques J P, Susskind M M

Abstract excerpt

Natural antisense RNAs have stem-loop (hairpin) secondary structures that are important for their function. The sar antisense RNA of phage P22 is unusual: the 3' half of the molecule forms an extensive stem-loop, but potential structures for the 5' half are not predicted to be thermodynamically stable. We devised a novel method to determine the secondary structure of sar RNA by examining the electrophoretic...

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Identifiers

PMID: 2057355
DOI: 10.1093/nar/19.11.2971
PMCID: PMC328259

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