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Mutational Analysis of sRNA-mRNA Base Pairing by Electrophoretic Mobility Shift Assay.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2018

Lillebæk Eva Maria Sternkopf, Kallipolitis Birgitte Haahr

Abstract excerpt

Small regulatory RNAs (sRNAs) in bacteria often act by base pairing to mRNAs. Direct interactions between an sRNA and its target mRNA can be investigated by electrophoretic mobility shift assay. In this assay, regions engaged in base pairing are analyzed by introducing mutations in one of the RNAs that prevent sRNA-mRNA complex formation, followed by the introduction of complementary mutations in its partner RNA...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 29484593
DOI: 10.1007/978-1-4939-7634-8_10

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