Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Increasing the Activity of the High-Fidelity SpyCas9 Form in Yeast by Directed Mutagenesis of the PAM-Interacting Domain.

International journal of molecular sciences - 28 Dec 2023

Davletshin Artem I, Matveeva Anna A, Bachurin Stanislav S, Karpov Dmitry S, Garbuz David G

Abstract excerpt

CRISPR/Cas systems are used for genome editing, both in basic science and in biotechnology. However, CRISPR/Cas editors have several limitations, including insufficient specificity leading to "off-targets" and the dependence of activity on chromatin state. A number of highly specific Cas9 variants have now been obtained, but most of them are characterized by reduced activity on eukaryotic chromatin. We identified...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Amino Acids
Share to Chromatin

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Mutagenesis

Share to Mutation

Share to Saccharomyces cerevisiae

Source

Open in PubMed Open full text

Identifiers

PMID: 38203615
DOI: 10.3390/ijms25010444
PMCID: PMC10779060

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Increasing the Activity of the High-Fidelity SpyCas9 Form in Yeast by Directed Mutagenesis of the PAM-Interacting Domain.PMID 38203615Read ↗

Engineered CRISPR-Cas9 nucleases with altered PAM specificities.Semantic proximity Read ↗

Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants.Semantic proximity Read ↗

Engineering a PAM-flexible SpdCas9 variant as a universal gene repressor.Semantic proximity Read ↗

Divergent PAM Specificity of a Highly-Similar SpCas9 OrthologSemantic proximity Read ↗

High-Throughput Screens of PAM-Flexible Cas9 Variants for Gene Knockout and Transcriptional Modulation.Semantic proximity Read ↗

PpCas9 from Pasteurella pneumotropica — a compact Type II-C Cas9 ortholog active in human cellsSemantic proximity Read ↗

A Cas9 with Complete PAM Recognition for Adenine DinucleotidesSemantic proximity Read ↗

Diverse noncanonical PAMs recognized by SpCas9 in human cellsSemantic proximity Read ↗

Evolved Cas9 variants with broad PAM compatibility and high DNA specificity.Semantic proximity Read ↗

Engineering of a high-fidelity Cas12a nuclease variant capable of allele-specific editing.Semantic proximity Read ↗

High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects.Semantic proximity Read ↗

Structural Basis for the Altered PAM Specificities of Engineered CRISPR-Cas9.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.