Article
Deciphering protein mutation-phenotype linkages from CRISPR-based tiling mutagenesis screens.
Cell systems - 19 Aug 2026
He Wei, Huang Jen-Wei, Wang Yalong, Hayward Samuel B, Leuzzi Giuseppe, Fu Rongjie, Wang Shuyue, Vaitsiankova Alina, Chen Yiwen, Bedford Mark T, Guerois Raphael, Ciccia Alberto, Xu Han
Abstract excerpt
CRISPR-based high-throughput mutagenesis screens enable systematic mapping of mutations to phenotypes, yet deciphering mutation-phenotype links remains challenging. Here, we present ProTiler-Mut, a versatile computational framework that leverages tiling mutagenesis screens, which introduce variants across entire protein sequences, to analyze mutation effects at the levels of residues, substructures, and...
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