Article
Efficient gene editing in induced pluripotent stem cells enabled by an inducible adenine base editor with tunable expression.
Scientific reports - 11 Dec 2023
Nandy Krittika, Babu Dinesh, Rani Sonam, Joshi Gaurav, Ijee Smitha, George Anila, Palani Dhavapriya, Premkumar Chitra, Rajesh Praveena, Vijayanand S, David Ernest, Murugesan Mohankumar, Velayudhan Shaji R
Abstract excerpt
The preferred method for disease modeling using induced pluripotent stem cells (iPSCs) is to generate isogenic cell lines by correcting or introducing pathogenic mutations. Base editing enables the precise installation of point mutations at specific genomic locations without the need for deleterious double-strand breaks used in the CRISPR-Cas9 gene editing methods. We created a bulk population of iPSCs that...
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