Article
Efficient generation of mouse models of human diseases via ABE- and BE-mediated base editing.
Nature communications - 14 Jun 2018
Liu Zhen, Lu Zongyang, Yang Guang, Huang Shisheng, Li Guanglei, Feng Songjie, Liu Yajing, Li Jianan, Yu Wenxia, Zhang Yu, Chen Jia, Sun Qiang, Huang Xingxu
Abstract excerpt
A recently developed adenine base editor (ABE) efficiently converts A to G and is potentially useful for clinical applications. However, its precision and efficiency in vivo remains to be addressed. Here we achieve A-to-G conversion in vivo at frequencies up to 100% by microinjection of ABE mRNA together with sgRNAs. We then generate mouse models harboring clinically relevant mutations at Ar and Hoxd13, which...
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