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Article

Homozygous gene disruption in diploid yeast through a single transformation.

Journal of bioscience and bioengineering - 1 Jan 2024

Kobashi Yuki, Nakayama Eri, Fukumori Naoki, Shimojima Ayane, Tabira Manami, Nishimura Yuki, Mukae Manami, Muto Ai, Nakashima Naoto, Okutsu Kayu, Yoshizaki Yumiko, Futagami Taiki, Takamine Kazunori, Tamaki Hisanori

Abstract excerpt

As industrial shochu yeast is a diploid strain, obtaining a strain with mutations in both allelic genes was considered difficult. We investigated a method for disrupting two copies of a homozygous gene with a single transformation. We designed a disruption cassette containing an intact LYS5 flanked by nonfunctional ura3 gene fragments divided into the 5'- and 3'-regions. These fragments had overlapping sequences...

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Identifiers

PMID: 37981488
DOI: 10.1016/j.jbiosc.2023.11.003

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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