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Article

Precise gene disruption in Saccharomyces cerevisiae by double fusion polymerase chain reaction.

Yeast (Chichester, England) - 1 Oct 1995

Amberg D C, Botstein D, Beasley E M

Abstract excerpt

We adapted a fusion polymerase chain reaction (PCR) strategy to synthesize gene disruption alleles of any sequenced yeast gene of interest. The first step of the construction is to amplify sequences flanking the reading frame we want to disrupt and to amplify the selectable marker sequence. Then we fuse the upstream fragment to the marker sequence by fusion PCR, isolate this product and fuse it to the downstream...

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PMID: 8553698
DOI: 10.1002/yea.320111307

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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