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Investigating Pseudomonas aeruginosa Gene Function During Pathogenesis Using Mobile-CRISPRi.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2024

Yu Michelle A, Banta Amy B, Ward Ryan D, Prasad Neha K, Kwon Michael S, Rosenberg Oren S, Peters Jason M

Abstract excerpt

CRISPR interference (CRISPRi) is a robust gene silencing technique that is ideal for targeting essential and conditionally essential (CE) genes. CRISPRi is especially valuable for investigating gene function in pathogens such as P. aeruginosa where essential and CE genes underlie clinically important phenotypes such as antibiotic susceptibility and virulence. To facilitate the use of CRISPRi in diverse...

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PMID: 37819512
DOI: 10.1007/978-1-0716-3473-8_2

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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