PAM-flexible Engineered FnCas9 variants for robust and ultra-precise genome editing and diagnostics.

doi:10.1038/s41467-024-49233-w

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Article

PAM-flexible Engineered FnCas9 variants for robust and ultra-precise genome editing and diagnostics.

Nature communications - 28 Jun 2024

Acharya Sundaram, Ansari Asgar Hussain, Kumar Das Prosad, Hirano Seiichi, Aich Meghali, Rauthan Riya, Mahato Sudipta, Maddileti Savitri, Sarkar Sajal, Kumar Manoj, Phutela Rhythm, Gulati Sneha, Rahman Abdul, Goel Arushi, Afzal C, Paul Deepanjan, Agrawal Trupti, Pulimamidi Vinay Kumar, Jalali Subhadra, Nishimasu Hiroshi, Mariappan Indumathi, Nureki Osamu, Maiti Souvik, Chakraborty Debojyoti

Abstract excerpt

The clinical success of CRISPR therapies hinges on the safety and efficacy of Cas proteins. The Cas9 from Francisella novicida (FnCas9) is highly precise, with a negligible affinity for mismatched substrates, but its low cellular targeting efficiency limits therapeutic use. Here, we rationally engineer the protein to develop enhanced FnCas9 (enFnCas9) variants and broaden their accessibility across human genomic...

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Identifiers

PMID: 38942756
DOI: 10.1038/s41467-024-49233-w
PMCID: PMC11213958

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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