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Article

Homology Directed Knockin of Point Mutations in the Zebrafish tardbp and fus Genes in ALS Using the CRISPR/Cas9 System.

PloS one - 1 Jan 2016

Armstrong Gary Alan Barclay, Liao Meijiang, You Zhipeng, Lissouba Alexandra, Chen Brian Edwin, Drapeau Pierre

Abstract excerpt

The methodology for site-directed editing of single nucleotides in the vertebrate genome is of considerable interest for research in biology and medicine. The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 type II (Cas9) system has emerged as a simple and inexpensive tool for editing genomic loci of interest in a variety of animal models. In zebrafish, error-prone...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 26930076
DOI: 10.1371/journal.pone.0150188
PMCID: PMC4773037

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