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Article

Guide-specific loss of efficiency and off-target reduction with Cas9 variants.

Nucleic acids research - 13 Oct 2023

Zhang Liang, He Wei, Fu Rongjie, Wang Shuyue, Chen Yiwen, Xu Han

Abstract excerpt

High-fidelity clustered regularly interspaced palindromic repeats (CRISPR)-associated protein 9 (Cas9) variants have been developed to reduce the off-target effects of CRISPR systems at a cost of efficiency loss. To systematically evaluate the efficiency and off-target tolerance of Cas9 variants in complex with different single guide RNAs (sgRNAs), we applied high-throughput viability screens and a synthetic...

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Identifiers

PMID: 37615574
DOI: 10.1093/nar/gkad702
PMCID: PMC10570041

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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