Article

Amplification-free long read sequencing reveals unforeseen CRISPR-Cas9 off-target activity

2020-02-09

Abstract excerpt

A much-debated concern about CRISPR-Cas9 genome editing is that unspecific guide RNA (gRNA) binding may induce off-target mutations. However, accurate prediction of CRISPR-Cas9 off-target sites and activity is challenging. Here we present SMRT-OTS and Nano-OTS, two amplification-free long-read sequencing protocols for detection of gRNA driven digestion of genomic DNA by Cas9. The methods were assessed using the hu...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 87068d7e-953e-5589-aa91-f34d4d026661
DOI: 10.1101/2020.02.09.940486

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Amplification-free long read sequencing reveals unforeseen CRISPR-Cas9 off-target activityDOI 10.1101/2020.02.09.940486Read

Amplification-free long-read sequencing reveals unforeseen CRISPR-Cas9 off-target activity.Semantic proximity Read

COSMID: A Web-based Tool for Identifying and Validating CRISPR/Cas Off-target SitesSemantic proximity Read

Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells.Semantic proximity Read

Massively Targeted Evaluation of Therapeutic CRISPR Off-Targets in CellsSemantic proximity Read

Latest Developed Strategies to Minimize the Off-Target Effects in CRISPR-Cas-Mediated Genome EditingSemantic proximity Read

<i>In vivo</i> CRISPR-Cas gene editing with no detectable genome-wide off-target mutationsSemantic proximity Read

Biased and Unbiased Methods for the Detection of Off-Target Cleavage by CRISPR/Cas9: An OverviewSemantic proximity Read

Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases.Semantic proximity Read

Off-target effects in CRISPR/Cas9 gene editingSemantic proximity Read

Unintended CRISPR-Cas9 editing outcomes: a review of the detection and prevalence of structural variants generated by gene-editing in human cellsSemantic proximity Read

Unbiased whole genome detection of ultrarare off-target mutations in genome-edited cell populations by HiFi sequencing.Semantic proximity Read

Human genetic variation alters CRISPR-Cas9 on- and off-targeting specificity at therapeutically implicated loci.Semantic proximity Read