Article
Generation of sheep with defined FecBB and TBXT mutations and porcine blastocysts with KCNJ5G151R/+ mutation using prime editing.
BMC genomics - 12 Jun 2023
Zhou Shiwei, Lenk Laura Johanna, Gao Yawei, Wang Yuhui, Zhao Xiaoe, Pan Menghao, Huang Shuhong, Sun Kexin, Kalds Peter, Luo Qi, Lillico Simon, Sonstegard Tad, Scholl Ute I, Ma Baohua, Petersen Bjoern, Chen Yulin, Wang Xiaolong
Abstract excerpt
BACKGROUND: Rewriting the genomes of living organisms has been a long-standing aim in the biological sciences. The revelation of the CRISPR/Cas9 technology has revolutionized the entire biological field. Since its emergence, this technology has been widely applied to induce gene knockouts, insertions, deletions, and base substitutions. However, the classical version of this system was imperfect for inducing or...
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