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Article

[Single base editing system mediates site-directed mutagenesis of genes GDF9 and FecB in Ouler Tibetan sheep].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology - 25 Jan 2023

Zhao Yifan, Zhang Yingbing, Yu Ruiluan, Wu Ying, Chen Yongzhong, Zhao Ruolin, Zhang Chengtu, Su Jianmin

Abstract excerpt

In this study, a single base editing system was used to edit the FecB and GDF9 gene to achieve a targeted site mutation from A to G and from C to T in Ouler Tibetan sheep fibroblasts, and to test its editing efficiency. Firstly, we designed and synthesized sgRNA sequences targeting FecB and GDF9 genes of Ouler Tibetan sheep, followed by connection to epi-ABEmax and epi-BE4max plasmids to construct vectors and...

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Identifiers

PMID: 36738211
DOI: 10.13345/j.cjb.220427

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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