Prolonging genetic circuit stability through adaptive evolution of overlapping genes.

Abstract excerpt

The development of synthetic biological circuits that maintain functionality over application-relevant time scales remains a significant challenge. Here, we employed synthetic overlapping sequences in which one gene is encoded or 'entangled' entirely within an alternative reading frame of another gene. In this design, the toxin-encoding relE was entangled within ilvA, which encodes threonine deaminase, an enzyme...

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• Binding Sites
• Escherichia coli
• Genes, Overlapping
• Genetic Engineering
• Mutation