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Article

Compartmentalized partnered replication for the directed evolution of genetic parts and circuits.

Nature protocols - 1 Dec 2017

Abil Zhanar, Ellefson Jared W, Gollihar Jimmy D, Watkins Ella, Ellington Andrew D

Abstract excerpt

Compartmentalized partnered replication (CPR) is an emulsion-based directed evolution method based on a robust and modular phenotype-genotype linkage. In contrast to other in vivo directed evolution approaches, CPR largely mitigates host fitness effects due to a relatively short expression time of the gene of interest. CPR is based on gene circuits in which the selection of a 'partner' function from a library...

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Identifiers

PMID: 29120463
DOI: 10.1038/nprot.2017.119
PMCID: PMC6053311

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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