Article

AI-directed gene fusing prolongs the evolutionary half-life of synthetic gene circuits

2025-02-12

Abstract excerpt

Evolutionary instability is a persistent challenge in synthetic biology, often leading to the loss of heterologous gene expression over time. Here, we present STABLES, a novel gene fusion strategy that links a gene of interest (GOI) to an essential endogenous gene (EG), with a “leaky” stop codon in between. This ensures both selective pressure against deleterious mutations and high expression of the GOI. By levera...

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Literature Corpus work: 04bceae2-2bcd-58ef-ab8c-c250c748c995
DOI: 10.1101/2025.02.10.637495

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