Engineering gene overlaps to sustain genetic constructs in vivo.

Abstract excerpt

Evolution is often an obstacle to the engineering of stable biological systems due to the selection of mutations inactivating costly gene circuits. Gene overlaps induce important constraints on sequences and their evolution. We show that these constraints can be harnessed to increase the stability of costly genes by purging loss-of-function mutations. We combine computational and synthetic biology approaches to...

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• Algorithms
• Escherichia coli
• Evolution, Molecular
• Genes, Essential
• Genetic Engineering