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Boosting genome editing efficiency in human cells and plants with novel LbCas12a variants

30 Apr 2023

Abstract excerpt

Abstract Background Cas12a (formerly known as Cpf1), the class II type V CRISPR nuclease, has been widely used for genome editing in mammalian cells and plants due to its distinct characteristics from Cas9. Despite being one of the most robust Cas12a nucleases, LbCas12a in general is less efficient than SpCas9 for genome editing in human cells, animals, and plants. Results To improve the editing efficiency of...

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PMID: 37122009
DOI: 10.1186/s13059-023-02929-6

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