Article
Expanding the scope of plant genome engineering with Cas12a orthologs and highly multiplexable editing systems.
Nature communications - 29 Mar 2021
Zhang Yingxiao, Ren Qiurong, Tang Xu, Liu Shishi, Malzahn Aimee A, Zhou Jianping, Wang Jiaheng, Yin Desuo, Pan Changtian, Yuan Mingzhu, Huang Lan, Yang Han, Zhao Yuxin, Fang Qing, Zheng Xuelian, Tian Li, Cheng Yanhao, Le Ysa, McCoy Bailey, Franklin Lidiya, Selengut Jeremy D, Mount Stephen M, Que Qiudeng, Zhang Yong, Qi Yiping
Abstract excerpt
CRISPR-Cas12a is a promising genome editing system for targeting AT-rich genomic regions. Comprehensive genome engineering requires simultaneous targeting of multiple genes at defined locations. Here, to expand the targeting scope of Cas12a, we screen nine Cas12a orthologs that have not been demonstrated in plants, and identify six, ErCas12a, Lb5Cas12a, BsCas12a, Mb2Cas12a, TsCas12a and MbCas12a, that possess...
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