Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

CRISPR-Cas12a exhibits metal-dependent specificity switching

1 Jul 2024

Abstract excerpt

Cas12a is the immune effector of type V-A CRISPR-Cas systems and has been co-opted for genome editing and other biotechnology tools. The specificity of Cas12a has been the subject of extensive investigation both in vitro and in genome editing experiments. However, in vitro studies have often been performed at high magnesium ion concentrations that are inconsistent with the free Mg2+ concentrations that would be...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to CRISPR and Genetic Engineering
Share to Cytomegalovirus and herpesvirus research
Share to Mosquito-borne diseases and control

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Identifiers

PMID: 39019776
DOI: 10.1093/nar/gkae613

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

CRISPR-Cas12a exhibits metal-dependent specificity switchingPMID 39019776Read ↗

Cas12e orthologs evolve variable structural elements to facilitate dsDNA cleavageSemantic proximity Read ↗

Comparative characterization of Cas12a2 orthologs identifies high-activity nucleases for programmable cell eliminationSemantic proximity Read ↗

CRISPR-Cas effector specificity and cleavage site determine phage escape outcomes.Semantic proximity Read ↗

Structural basis for DNase activity of a conserved protein implicated in CRISPR-mediated genome defense.Semantic proximity Read ↗

CRISPR-Cas12a REC2 – NUC interactions drive target-strand cleavage and constrain trans cleavageSemantic proximity Read ↗

Two novel mgCas12a proteins from the human gut metagenome exhibit reduced collateral DNase activitySemantic proximity Read ↗

AcrIIA5 Inhibits a Broad Range of Cas9 Orthologs by Preventing DNA Target CleavageSemantic proximity Read ↗

Systematic in vitro profiling of off-target affinity, cleavage and efficiency for CRISPR enzymes.Semantic proximity Read ↗

Engineering salt-tolerant Cas12f1 variants for gene-editing applications.Semantic proximity Read ↗

Structural basis of Cas3 activation in type I-C CRISPR-Cas systemSemantic proximity Read ↗

Engineering a High-Fidelity MAD7 Variant with Enhanced Specificity for Precision Genome Editing via CcdB-Based Bacterial Screening.Semantic proximity Read ↗

Systematic decomposition of sequence determinants governing CRISPR/Cas9 specificity.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.