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Article

CRISPR-based large-scale modeling of loss-of-function mutations to investigate mechanisms of stress resistance in cancer.

STAR protocols - 17 Mar 2023

Simeoni Fabrizio, Loukas Ioannis, Wilson Thomas Stuart, Scaffidi Paola

Abstract excerpt

Dissecting mechanisms driving subclone expansion in primary cancers has been challenging. Here, we present a protocol to systematically disrupt entire gene networks and assess the functional impact of this perturbation on cancer cell fitness. By combining arrayed CRISPR libraries and high-content microscopy, we describe steps to identify classes of genes whose inactivation promotes resistance to environmental...

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Identifiers

PMID: 36853711
DOI: 10.1016/j.xpro.2023.102097
PMCID: PMC9947008

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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