Article
[Correction of the pathogenic mutation in the G6PC3 gene by adenine base editing in mutant embryos].
Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi - 14 Apr 2023
Hong M, Wang P, Shangguan T, Li G L, Bian R P, He W, Jiang W, Chen J P
Abstract excerpt
Objective: To determine whether the adenine base editor (ABE7.10) can be used to fix harmful mutations in the human G6PC3 gene. Methods: To investigate the safety of base-edited embryos, off-target analysis by deep sequencing was used to examine the feasibility and editing efficiency of various sgRNA expression vectors. The human HEK293T mutation models and human embryos were also used to test the feasibility and...
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