Article
Necessity of integrated genomic analysis to establish a designed knock-in mouse from CRISPR-Cas9-induced mutants.
Scientific reports - 27 Nov 2022
Yoshida Masahide, Saito Tomoko, Takayanagi Yuki, Totsuka Yoshikazu, Onaka Tatsushi
Abstract excerpt
The CRISPR-Cas9 method for generation of knock-in mutations in rodent embryos yields many F0 generation candidates that may have the designed mutations. The first task for selection of promising F0 generations is to analyze genomic DNA which likely contains a mixture of designed and unexpected mutations. In our study, while generating Prlhr-Venus knock-in reporter mice, we found that genomic rearrangements near...
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