Article
Effect of mutations at the lariat branch acceptor site on beta-globin pre-mRNA splicing in vitro.
Nature - 1 Jan 2000
Hornig H, Aebi M, Weissmann C
Abstract excerpt
Introns are excised from full-length transcripts (pre-messenger RNAs) of eukaryotic genes in two steps. First, the pre-mRNA is cleaved at the 5' splice site and a branched (lariat) intermediate is formed. Then, cleavage at the 3' splice site and ligation of the two exons leads to the release of the lariat intron. The intron sequence which accepts the 5' end to form the lariat branch is strictly conserved in...
Topics
- Adenine Nucleotides
- Animals
- Base Sequence
- Cytosine Nucleotides
- Exons
- Globins
- Guanine Nucleotides
- Introns
- Mutation
- Nucleic Acid Precursors
- RNA Precursors
- RNA Splicing