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Article

Unexpected point mutations activate cryptic 3' splice sites by perturbing a natural secondary structure within a yeast intron.

Genes & development - 1 Jul 1991

Deshler J O, Rossi J J

Abstract excerpt

The 3' splice site of the budding yeast Kluyveromyces lactis actin gene (ACT) intron is distally spaced (122 nucleotides) from its branchpoint and is also preceded by a silent PyAG located 43 nucleotides upstream. We devised a genetic screen that resulted in the isolation of several randomly induced cis-acting mutations that activate the silent PyAG as a 3' splice site. These mutations fall within a region...

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PMID: 2065975
DOI: 10.1101/gad.5.7.1252

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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