Article
Unexpected point mutations activate cryptic 3' splice sites by perturbing a natural secondary structure within a yeast intron.
Genes & development - 1 Jul 1991
Deshler J O, Rossi J J
Abstract excerpt
The 3' splice site of the budding yeast Kluyveromyces lactis actin gene (ACT) intron is distally spaced (122 nucleotides) from its branchpoint and is also preceded by a silent PyAG located 43 nucleotides upstream. We devised a genetic screen that resulted in the isolation of several randomly induced cis-acting mutations that activate the silent PyAG as a 3' splice site. These mutations fall within a region...
Topics
- Base Composition
- Base Sequence
- Gene Expression Regulation, Fungal
- Introns
- Kluyveromyces
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- RNA Splicing
- RNA, Messenger
- Saccharomyces cerevisiae