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Article

The conserved dinucleotide AG of the 3' splice site may be recognized twice during in vitro splicing of mammalian mRNA precursors.

Gene - 15 Jun 1990

Zhuang Y, Weiner A M

Abstract excerpt

We have previously used site-directed mutagenesis to introduce an additional branch site into the first intron of the human beta-globin gene at nt -24 between the natural branch site (nt -37) and the normal 3' splice site (nt -1). We found that either the upstream or downstream branch site could be used during in vitro splicing, depending on which site best matched the mammalian branch site consensus YURAC (R =...

Topics

Adenine
Base Sequence
Exons
Globins
Guanine
Humans
In Vitro Techniques
Introns
Molecular Sequence Data
Mutation
RNA Precursors
RNA Splicing

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Identifiers

PMID: 2401404
DOI: 10.1016/0378-1119(90)90189-x

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2401404-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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The conserved dinucleotide AG of the 3' splice site may be recognized twice during in vitro splicing of mammalian mRNA precursors.PMID 2401404Read ↗

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