Article
Accumulation of a novel spliceosomal complex on pre-mRNAs containing branch site mutations.
Molecular and cellular biology - 1 Oct 1995
Champion-Arnaud P, Gozani O, Palandjian L, Reed R
Abstract excerpt
Pre-mRNA assembles into spliceosomal complexes in the stepwise pathway E-->A-->B-->C. We show that mutations in the metazoan branchpoint sequence (BPS) have no apparent effect on E complex formation but block the assembly of the A complex and the UV cross-linking of U2 small nuclear ribonucleoprotein particle (snRNP) proteins. Unexpectedly, a novel complex, designated E*, assembles on pre-mRNAs containing BPS...
Topics
- Base Sequence
- Molecular Sequence Data
- Mutation
- Nuclear Proteins
- RNA Precursors
- RNA Splicing
- RNA, Messenger
- RNA, Small Nuclear
- Ribonucleoprotein, U1 Small Nuclear
- Ribonucleoprotein, U2 Small Nuclear
- Ribonucleoproteins