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Article

High-Fidelity Cytosine Base Editing in a GC-Rich Corynebacterium glutamicum with Reduced DNA Off-Target Editing Effects.

Microbiology spectrum - 21 Dec 2022

Heo Yu Been, Hwang Gue-Ho, Kang Seok Won, Bae Sangsu, Woo Han Min

Abstract excerpt

Genome editing technology is a powerful tool for programming microbial cell factories. However, rat APOBEC1-derived cytosine base editor (CBE) that converts C•G to T•A at target genes induced DNA off-targets, regardless of single-guide RNA (sgRNA) sequences. Although the high efficiencies of the bacterial CBEs have been developed, a risk of unidentified off-targets impeded genome editing for microbial cell...

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Identifiers

PMID: 36374037
DOI: 10.1128/spectrum.03760-22
PMCID: PMC9769817

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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