Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Base editor enables rational genome-scale functional screening for enhanced industrial phenotypes in Corynebacterium glutamicum.

Science advances - 2 Sept 2022

Liu Ye, Wang Ruoyu, Liu Jiahui, Lu Hui, Li Haoran, Wang Yu, Ni Xiaomeng, Li Junwei, Guo Yanmei, Ma Hongwu, Liao Xiaoping, Wang Meng

Abstract excerpt

Genome-scale functional screening accelerates comprehensive assessment of gene function in cells. Here, we have established a genome-scale loss-of-function screening strategy that combined a cytosine base editor with approximately 12,000 parallel sgRNAs targeting 98.1% of total genes in Corynebacterium glutamicum ATCC 13032. Unlike previous data processing methods developed in yeast or mammalian cells, we...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Corynebacterium glutamicum
Share to Gene Editing

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Genomics

Share to Phenotype

Source

Open in PubMed Open full text

Identifiers

PMID: 36044571
DOI: 10.1126/sciadv.abq2157
PMCID: PMC9432829

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Base editor enables rational genome-scale functional screening for enhanced industrial phenotypes in Corynebacterium glutamicum.PMID 36044571Read ↗

Base editor-mediated large-scale screening of functional mutations in bacteria for industrial phenotypes.Semantic proximity Read ↗

High-Fidelity Cytosine Base Editing in a GC-Rich Corynebacterium glutamicum with Reduced DNA Off-Target Editing Effects.Semantic proximity Read ↗

Annotation and evaluation of base editing outcomes in multiple cell types using CRISPRbase.Semantic proximity Read ↗

Single-Base Genome Editing in Corynebacterium glutamicum with the Help of Negative Selection by Target-Mismatched CRISPR/Cpf1.Semantic proximity Read ↗

Genome-wide interrogation of gene functions through base editor screens empowered by barcoded sgRNAsSemantic proximity Read ↗

Mobile-CRISPRi: Enabling Genetic Analysis of Diverse BacteriaSemantic proximity Read ↗

High-throughput transposon mutagenesis of Corynebacterium glutamicum and construction of a single-gene disruptant mutant library.Semantic proximity Read ↗

Identification of functional sgRNA mutants lacking canonical secondary structure using high-throughput FACS screening.Semantic proximity Read ↗

Pooled CRISPR screening enables genotype-phenotype association in industrial microorganisms.Semantic proximity Read ↗

One-for-all gene inactivation via PAM-independent base editing in bacteriaSemantic proximity Read ↗

Genome-wide mapping of mutations at single-nucleotide resolution for protein, metabolic and genome engineering.Semantic proximity Read ↗

Modular (de)construction of complex bacterial phenotypes by CRISPR/nCas9-assisted, multiplex cytidine base-editing.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.