Article
Efficient Gene Silencing by Adenine Base Editor-Mediated Start Codon Mutation.
Molecular therapy : the journal of the American Society of Gene Therapy - 5 Feb 2020
Wang Xinjie, Liu Zhiwei, Li GuangLei, Dang Lu, Huang Shisheng, He Lei, Ma Yu'e, Li Cong, Liu Ming, Yang Guang, Huang Xingxu, Zhou Fei, Ma Xiaodong
Abstract excerpt
Traditional CRISPR/Cas9-based gene knockouts are generated by introducing DNA double-strand breaks (DSBs), but this may cause excessive DNA damage or cell death. CRISPR-based cytosine base editors (CBEs) and adenine base editors (ABEs) can facilitate C-to-T or A-to-G exchanges, respectively, without DSBs. CBEs have been employed in a gene knockout strategy: CRISPR-STOP or i-STOP changes single nucleotides to...
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