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Article

High-throughput transposon mutagenesis of Corynebacterium glutamicum and construction of a single-gene disruptant mutant library.

Applied and environmental microbiology - 1 May 2006

Suzuki Nobuaki, Okai Naoko, Nonaka Hiroshi, Tsuge Yota, Inui Masayuki, Yukawa Hideaki

Abstract excerpt

A simple and high-throughput transposon-mediated mutagenesis system employing two different types of transposons in combination with direct genomic DNA amplification and thermal asymmetric interlaced PCR (TAIL-PCR) was developed. Each of the two minitransposons based on IS31831 (ISL3 family) and Tn5 (IS4 family) was integrated into the Corynebacterium glutamicum R genome. By using BLAST and Perl, transposon...

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Identifiers

PMID: 16672528
DOI: 10.1128/AEM.72.5.3750-3755.2006
PMCID: PMC1472376

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Source receipt: pubmed, retrieved 7 Sept 2026.

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