Article
Cytosine Base Editing in Bacteria.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2023
Liu Ye, Liu Yang, Zheng Ping, Wang Yu, Wang Meng
Abstract excerpt
Base editing is a new genome editing technology that enables DNA base mutations without requiring double-stranded DNA backbone cleavage or a donor template. It has been widely used for genome engineering of eukaryotic and prokaryotic microorganisms. In this chapter, we describe a routine protocol for cytosine base editing in two model bacteria Corynebacterium glutamicum and Bacillus subtilis. The protocol can be...
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