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Article

Applying a Knock-In Strategy to Create Reporter-Tagged Knockout Alleles in Axolotls.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2023

Wang Liqun, Zeng Yan-Yun, Liu Yanmei, Fei Ji-Feng

Abstract excerpt

Tetrapod species axolotls exhibit the powerful capacity to fully regenerate their tail and limbs upon injury, hence serving as an excellent model organism in regenerative biology research. Developing proper molecular and genetic tools in axolotls is an absolute necessity for deep dissection of tissue regeneration mechanisms. Previously, CRISPR-/Cas9-based knockout and targeted gene knock-in approaches have been...

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PMID: 36272087
DOI: 10.1007/978-1-0716-2659-7_23

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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