Article

Rapid and precise genome engineering in a naturally short-lived vertebrate

2022-05-26

Abstract excerpt

The African turquoise killifish is a powerful vertebrate system to study complex phenotypes at scale, including aging and age-related disease. Here we develop a rapid and precise CRISPR/Cas9-mediated knock-in approach in the killifish. We show its efficient application to precisely insert fluorescent reporters of different sizes at various genomic loci, to drive cell-type- and tissue-specific expression. This knoc...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: b8f40174-538d-5f34-9a20-2bb97cf381c3
DOI: 10.1101/2022.05.25.493454

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Rapid and precise genome engineering in a naturally short-lived vertebrateDOI 10.1101/2022.05.25.493454Read

Rapid reverse genetics systems for N. furzeri, a suitable model organism to study vertebrate agingSemantic proximity Read

Rapid reverse genetics systems for Nothobranchius furzeri, a suitable model organism to study vertebrate agingSemantic proximity Read

The African Turquoise Killifish: A Model for Exploring Vertebrate Aging and Diseases in the Fast Lane.Semantic proximity Read

The African Turquoise Killifish: A Scalable Vertebrate Model for Aging and Other Complex Phenotypes.Semantic proximity Read

One-step generation of complete gene knockout mice and monkeys by CRISPR/Cas9-mediated gene editing with multiple sgRNAs.Semantic proximity Read

Targeted genome editing in primate embryos.Semantic proximity Read

A simple and efficient method to visualize and quantify the efficiency of chromosomal mutations from genome editing.Semantic proximity Read

The hitchhiker's guide to Xenopus genetics.Semantic proximity Read

Endogenous protein tagging in medaka using a simplified CRISPR/Cas9 knock-in approachSemantic proximity Read

CRIMP: a CRISPR/Cas9 insertional mutagenesis protocol and toolkit.Semantic proximity Read

Manipulation of the Tyrosinase gene permits improved CRISPR/Cas editing and neural imaging in cichlid fish.Semantic proximity Read

Genome-scale CRISPR screening in a single mouse liverSemantic proximity Read