Article
Generating and identifying axolotls with targeted mutations using Cas9 RNA-guided nuclease.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2015
Flowers G Parker, Crews Craig M
Abstract excerpt
The CRISPR/Cas9 RNA-guided nuclease now enables a reverse genetics approach to investigate the function of genes of interest during regeneration in the axolotl. The process of generating the constructs necessary for targeting a gene of interest is considerably less labor intensive than for other methods of targeted mutagenesis such as Zinc finger nucleases or Transcription activator-like effector nucleases. Here,...
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