Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

An easy-to-use site-directed mutagenesis method with a designed restriction site for convenient and reliable mutant screening.

Journal of Zhejiang University. Science. B - 1 Jun 2009

Zhang Bao-zhong, Zhang Xin, An Xiao-ping, Ran Duo-liang, Zhou Yu-sen, Lu Jun, Tong Yi-gang

Abstract excerpt

Site-directed mutagenesis (SDM) has been a very important method to probe the function-structure relationship of proteins. In this study, we introduced an easy-to-use, polymerase chain reaction (PCR)-based SDM method for double-stranded plasmid DNA, with a designed restriction site to ensure simple and efficient mutant screening. The DNA sequence to be mutated was first translated into amino acid sequence and...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Escherichia coli
Share to Escherichia coli Proteins

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Genetic Testing

Share to Mutagenesis, Site-Directed

Share to Mutation

Share to Polymerase Chain Reaction

Source

Open in PubMed Open full text

Identifiers

PMID: 19489114
DOI: 10.1631/jzus.B0820367
PMCID: PMC2689561

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

An easy-to-use site-directed mutagenesis method with a designed restriction site for convenient and reliable mutant screening.PMID 19489114Read ↗

Improved site-directed mutagenesis method using PCR.Semantic proximity Read ↗

A site-directed mutagenesis method particularly useful for creating otherwise difficult-to-make mutants and alanine scanning.Semantic proximity Read ↗

Highly efficient and simple SSPER and rrPCR approaches for the accurate site-directed mutagenesis of large and small plasmids.Semantic proximity Read ↗

Site-directed mutagenesis.Semantic proximity Read ↗

An In Vitro Single-Primer Site-Directed Mutagenesis Method for Use in Biotechnology.Semantic proximity Read ↗

Multiplex site-directed mutagenesis strategy including high-efficiency selection of the mutant PCR products.Semantic proximity Read ↗

[A simple and convenient method for construction of gene site-directed mutagenesis].Semantic proximity Read ↗

Efficiency and Fidelity of Site-Directed Mutagenesis with Complementary Primer Pairs.Semantic proximity Read ↗

A new method for multi-site-directed mutagenesis.Semantic proximity Read ↗

TAMS technology for simple and efficient in vitro site-directed mutagenesis and mutant screening.Semantic proximity Read ↗

[A method for introducing mutations into large vectors].Semantic proximity Read ↗

Inverse PCR for Site-Directed Mutagenesis.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.