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Efficiency and Fidelity of Site-Directed Mutagenesis with Complementary Primer Pairs.

Cells - 13 Jan 2026

Varela-Castillo Paulina, Razavi Arezousadat, Mousavi Negar, Robinson Nicole, Yang Xiang-Jiao

Abstract excerpt

Based on PCR with complementary primer pairs and Pfu DNA polymerase, QuickChange site-directed mutagenesis has been widely employed, but its efficiency varies from mutation to mutation. An alternative strategy relies on partially overlapping primer pairs with 3'-overhangs, and this strategy has led to the recent development of P3a and P3b site-directed mutagenesis, in which the use of SuperFi II and Q5...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 41597213
DOI: 10.3390/cells15020138
PMCID: PMC12839903

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GC

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026.

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