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Article

A gradient PCR-based screen for use in site-directed mutagenesis.

Analytical biochemistry - 15 Mar 2003

Padmakumar V C, Varadarajan Raghavan

Abstract excerpt

Site-directed mutagenesis is widely used to study protein and nucleic acid structure and function. Despite recent advancements in the efficiency of procedures for site-directed mutagenesis, the fraction of site-directed mutants by most procedures rarely exceeds 50% on a routine basis and is never 100%. Hence it is typically necessary to sequence two or three clones each time a site-directed mutant is constructed....

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PMID: 12654318
DOI: 10.1016/s0003-2697(02)00688-7

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Source receipt: pubmed, retrieved 7 Sept 2026.

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