Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

An In Vitro Single-Primer Site-Directed Mutagenesis Method for Use in Biotechnology.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2017

Huang Yanchao, Zhang Likui

Abstract excerpt

Site-directed mutagenesis is a powerful method to introduce mutation(s) into DNA sequences. A number of methods have been developed over the years with a main goal being to create a high number of mutant genes. The single-mutagenic primer method for site-directed mutagenesis is the most direct method that yields mutant genes in about 25-50 % of transformants in a robust, low-cost reaction. The supercompetent...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Bacteria
Share to Base Sequence
Share to Biotechnology

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to DNA-Directed DNA Polymerase

Share to DNA Primers

Share to Mutagenesis, Site-Directed

Share to Mutation

Share to Polymerase Chain Reaction

Source

Identifiers

PMID: 27709590
DOI: 10.1007/978-1-4939-6472-7_26

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

An In Vitro Single-Primer Site-Directed Mutagenesis Method for Use in Biotechnology.PMID 27709590Read ↗

Improved site-directed mutagenesis method using PCR.Semantic proximity Read ↗

TAMS technology for simple and efficient in vitro site-directed mutagenesis and mutant screening.Semantic proximity Read ↗

A general method for rapid site-directed mutagenesis using the polymerase chain reaction.Semantic proximity Read ↗

Highly efficient and simple SSPER and rrPCR approaches for the accurate site-directed mutagenesis of large and small plasmids.Semantic proximity Read ↗

A simple high-efficiency method for random mutagenesis of cloned genes using forced nucleotide misincorporation.Semantic proximity Read ↗

An easy-to-use site-directed mutagenesis method with a designed restriction site for convenient and reliable mutant screening.Semantic proximity Read ↗

Site-directed mutagenesis by the megaprimer PCR method: variations on a theme for simultaneous introduction of multiple mutations.Semantic proximity Read ↗

Simple and efficient oligonucleotide-directed mutagenesis using one primer and circular plasmid DNA template.Semantic proximity Read ↗

Efficiency and Fidelity of Site-Directed Mutagenesis with Complementary Primer Pairs.Semantic proximity Read ↗

DNA mutagenesis and recombination.Semantic proximity Read ↗

A site-directed mutagenesis method particularly useful for creating otherwise difficult-to-make mutants and alanine scanning.Semantic proximity Read ↗

A novel megaprimed and ligase-free, PCR-based, site-directed mutagenesis method.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.