Article
Error-prone rolling circle amplification: the simplest random mutagenesis protocol.
Nature protocols - 1 Jan 2006
Fujii Ryota, Kitaoka Motomitsu, Hayashi Kiyoshi
Abstract excerpt
A simple protocol to introduce random mutations, named error-prone rolling circle amplification (RCA), is described. A template plasmid is amplified by RCA in the presence of MnCl2 and used for transformation of a host strain to give a mutant library with three to four random point mutations per kilobase throughout the entire plasmid. The prime advantage of this method is its simplicity. This protocol requires...
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