A simple high-efficiency method for random mutagenesis of cloned genes using forced nucleotide misincorporation.

doi:10.1016/0378-1119(90)90066-z

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Article

A simple high-efficiency method for random mutagenesis of cloned genes using forced nucleotide misincorporation.

Gene - 30 Mar 1990

Liao X B, Wise J A

Abstract excerpt

We describe a simple protocol for introducing random point mutations into cloned DNA fragments via forced misincorporation of deoxynucleoside triphosphates (dNTPs) by either of two polymerases which lack proofreading activity, reverse transcriptase or a mutant T7 DNA polymerase. A high ratio of o...

Topics

Base Sequence
Cloning, Molecular
DNA Mutational Analysis
DNA-Directed DNA Polymerase
Molecular Sequence Data
Mutation
RNA-Directed DNA Polymerase
Transcription, Genetic

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