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Article

A simple high-efficiency method for random mutagenesis of cloned genes using forced nucleotide misincorporation.

Gene - 30 Mar 1990

Liao X B, Wise J A

Abstract excerpt

We describe a simple protocol for introducing random point mutations into cloned DNA fragments via forced misincorporation of deoxynucleoside triphosphates (dNTPs) by either of two polymerases which lack proofreading activity, reverse transcriptase or a mutant T7 DNA polymerase. A high ratio of one nucleotide to the other three is used to enhance the error rate. Mutagenesis is initiated from a specific primer and...

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Source

Identifiers

PMID: 1692798
DOI: 10.1016/0378-1119(90)90066-z

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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