Frequency and mechanisms of LINE-1 retrotransposon insertions at CRISPR/Cas9 sites

Abstract excerpt

CRISPR/Cas9-based genome editing has revolutionized experimental molecular biology and entered the clinical world for targeted gene therapy. Identifying DNA modifications occurring at CRISPR/Cas9 target sites is critical to determine efficiency and safety of editing tools. Here we show that insertions of LINE-1 (L1) retrotransposons can occur frequently at CRISPR/Cas9 editing sites. Together with PolyA-seq and an...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Chromosomal and Genetic Variations
• Share to CRISPR and Genetic Engineering
• Share to Cytomegalovirus and herpesvirus research