Article

Customizable host and viral transcript enrichment using CRISPR-Cas9 long-read sequencing for isoform discovery and validation

2025-04-14

Abstract excerpt

Long-read RNA sequencing has been broadly utilized to examine the diversity of transcriptomes, understand differential expression and discover novel transcript isoforms. One of the major limitations of whole transcriptome sequencing is the difficulty in obtaining sufficient depth for low abundant transcripts. Methods which address this are either difficult to scale or customize: long- range PCR is customizable but...

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Literature Corpus work: 9bb81b92-7c4b-5a8d-83a7-783a6f1b29c4
DOI: 10.1101/2025.04.11.648353

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