Article

Improved long-transcript representation in Oxford Nanopore direct RNA sequencing with UltraMarathonRT

2025-12-12

Abstract excerpt

While most RNA-seq methods sequence amplified cDNA molecules, the advent of direct RNA sequencing (DRS) empowered the scientific community to read native RNA. This technology unlocked characterization of natural RNA modifications and long RNA isoforms without the inherent biases of PCR amplification. In the library preparation prior to Oxford Nanopore (ONT) sequencing, polyadenylated RNAs are copied by a reverse t...

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Literature Corpus work: 6696def7-79fa-5d98-b088-90faf5c1a8b0
DOI: 10.64898/2025.12.10.693495

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